Transcription Factor GmERF105 Negatively Regulates Salt Stress Tolerance in Arabidopsis thaliana

The Ethylene Response Factor (ERF) transcription factors form a subfamily of the AP2/ERF family that is instrumental in mediating plant responses to diverse abiotic stressors. Herein, we present isolation and characterization GmERF105 gene from Williams 82 (W82), which rapidly induced by salt, drought, abscisic acid (ABA) treatments soybean. protein contains an AP2 domain localizes nucleus. was selectively bound GCC-box gel migration experiments. Under salt stress, overexpression Arabidopsis significantly reduced seed germination rate, fresh weight, antioxidant enzyme activity; meanwhile, sodium ion content, malonic dialdehyde (MDA) reactive oxygen species (ROS) levels were markedly elevated compared wild type. It further found CSD1 CDS2 two SOD genes OE lines. Furthermore, transgenic plants displayed suppressed expression stress response marker genes, including KIN1, LEA14, NCED3, RD29A, COR15A/B, under treatment. Our findings suggest can act as negative regulator tolerance pathways affecting ROS scavenging systems genes.